DCXR Polyclonal antibody

DCXR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15188-1-AP

Synonyms

Carbonyl reductase II, DCR, DCXR, HCR2, HCRII, KIDCR, Kidney dicarbonyl reductase, L xylulose reductase, P34H, SDR20C1, Sperm surface protein P34H, XR

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Validation Data Gallery

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  WB analysis using 15188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15188-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15188-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, human testis tissue, human liver tissue, mouse testis tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15188-1-AP targets DCXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCXR fusion protein Ag7272
• Full Name: dicarbonyl/L-xylulose reductase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001470
• Gene Symbol: DCXR
• Gene ID (NCBI): 51181
• RRID: AB_2088504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.