DCUN1D5 Polyclonal antibody

DCUN1D5 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14810-1-AP

Synonyms

DCN1 like protein 5, DCUN1D5

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Validation Data Gallery

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  WB analysis of HEK-293 using 14810-1-AP

  WB result of DCUN1D5 antibody (14810-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCUN1D5 transfected HEK-293 cells.

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  WB analysis of HeLa using 14810-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14810-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14810-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 14810-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14810-1-AP

  IP result of anti-DCUN1D5 (IP:14810-1-AP, 3ug; Detection:14810-1-AP 1:800) with HeLa cells lysate 1840ug.

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  IF Staining of HeLa using 14810-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14810-1-AP (DCUN1D5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse brain tissue, rat spleen tissue, U-937 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14810-1-AP targets DCUN1D5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCUN1D5 fusion protein Ag6534
• Full Name: DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae)
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC004169
• Gene Symbol: DCUN1D5
• Gene ID (NCBI): 84259
• RRID: AB_2088468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Conjugation of Nedd8 to a cullin protein, termed neddylation, is an evolutionarily conserved process that functions to activate the cullin-RING family E3 ubiquitin ligases, leading to increased proteasomal degradation of a wide range of substrate proteins. Cellular neddylation requires the action of DCN1 (Defective in cullin neddylation protein 1), which, in humans, consists of five homologues designated as DCNL1-5. DCUN1D5 gene encodes the 28 kDa DCUN1 domain-containing protein 5 containing a C-terminal potentiating neddylation domain. The cellular function of DCUN1D5 needs to be explored, recently, a mono-ubiquitination-mediated mechanism that governs nuclear-cytoplasmic trafficking of hDCNL1, was reported to regulate hDCNL1-dependent activation of the cullin-RING E3 ubiquitin ligases in selected cellular compartments.

Publications

Species	Application	Title
human	WB	Elife; SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.; Authors - Kate E Coleman
human	WB,IHC	Asian Pac J Cancer Prev; In vitro biological characterization of DCUN1D5 in DNA damage response.; Authors - Guo Wei W