DCTN4 Polyclonal antibody

• Featured Product
• KD/KO Validated

DCTN4 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17098-1-AP

Synonyms

DCTN4, dynactin 4 (p62), Dynactin subunit 4, Dynactin subunit p62

---

Validation Data Gallery

• 

  WB analysis of human brain using 17098-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17098-1-AP

  IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

---

Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17098-1-AP targets DCTN4 in WB, IP,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, Monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCTN4 fusion protein Ag10579
• Full Name: dynactin 4 (p62)
• Calculated Molecular Weight: 460 aa, 52 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC026323
• Gene Symbol: DCTN4
• Gene ID (NCBI): 51164
• RRID: AB_2276944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
Monkey	WB	Mol Biol Cell; Dynactin's pointed-end complex is a cargo-targeting module.; Authors - Ting-Yu Yeh ; KD Validated
human	WB	Onco Targets Ther; Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.; Authors - Xin Xing Li