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DCK Polyclonal antibody

DCK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17758-1-AP

Synonyms

DCK, deoxycytidine kinase



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCK fusion protein Ag12123
Full Name deoxycytidine kinase
Calculated Molecular Weight 260 aa, 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC103764
Gene Symbol DCK
Gene ID (NCBI) 1633
RRIDAB_2261476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).

Protocols

Product Specific Protocols
WB protocol for DCK antibody 17758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer.

Authors - Yaoyi Xiong
humanWB

Am J Cancer Res

PMID: 32368394

Authors - Zeng Ye
humanWB

Int J Cancer

Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.

Authors - Nao Yoshida-Sakai
humanWB

Front Oncol

m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.

Authors - Congjun Zhang
humanWB

Invest New Drugs

Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma.

Authors - Irene Dell'Anno
humanWB

Sci Rep

Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia.

Authors - Susan K Rathe
  • KO Validated