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CoraLite® Plus 488-conjugated DAXX Monoclonal antibody

DAXX Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F3G9

Cat no : CL488-67879

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated DAXX Monoclonal anti (CL488-67879)

    IF Staining of A431 using CL488-67879

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DAXX antibody (CL488-67879, Clone: 1F3G9 ) at dilution of 1:400.

Tested Applications

Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67879 targets DAXX in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DAXX fusion protein Ag14606
Full Name death-domain associated protein
Calculated Molecular Weight 740 aa, 81 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DAXX antibody CL488-67879Download protocol
Standard Protocols
Click here to view our Standard Protocols