DARS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DARS2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13807-1-AP

Synonyms

Aspartate tRNA ligase, ASPRS, DARS2, LBSL, MT ASPRS, RP3 383J4.2

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Validation Data Gallery

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  WB analysis using 13807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13807-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13807-1-AP (DARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13807-1-AP (DARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13807-1-AP (DARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13807-1-AP (DARS2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13807-1-AP (DARS2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13807-1-AP (DARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 13807-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (13807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, human placenta tissue, U-937 cells
• Positive IHC detected in: mouse cerebellum tissue, human brain tissue, human testis tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

13807-1-AP targets DARS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DARS2 fusion protein Ag4809
• Full Name: aspartyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 645 aa, 74 kDa
• Observed Molecular Weight: 66-74 kDa
• GenBank Accession Number: BC045173
• Gene Symbol: DARS2
• Gene ID (NCBI): 55157
• RRID: AB_2088759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing.; Authors - Sebastian Willenborg
mouse	WB	Cell Metab; Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart.; Authors - Sukru Anil Dogan
mouse	WB	EMBO Rep; Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.; Authors - Dominic Seiferling ; KO Validated
mouse	WB	Int J Biochem Cell Biol; Mitochondrial oxidative phosphorylation is impaired in TALLYHO mice, a new obesity and type 2 diabetes animal model.; Authors - Caroline A Hunter
human,mouse	IHC,WB	Thorac Cancer; DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway; Authors - Tao Fang ; KD Validated
human	IHC	J Transl Med; DARS2 overexpression is associated with PET/CT metabolic parameters and affects glycolytic activity in lung adenocarcinoma; Authors - Xu-Sheng Liu