DACH2 Polyclonal antibody

DACH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25219-1-AP

Synonyms

DACH2, Dachshund homolog 2

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Validation Data Gallery

• 

  WB analysis of mouse heart using 25219-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 25219-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 25219-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 25219-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25219-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse eye tissue, mouse kidney tissue, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25219-1-AP is DACH2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DACH2 fusion protein Ag18403
• Full Name: dachshund homolog 2 (Drosophila)
• Calculated Molecular Weight: 599 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC114950
• Gene Symbol: DACH2
• Gene ID (NCBI): 117154
• RRID: AB_2879967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dachshund homolog 2 (DACH2), is a 599 amino acid protein, which belongs to the DACH family. DACH2 localizes in the nucleus and as a transcription factor is involved in regulation of organogenesis.