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CYP20A1 Polyclonal antibody

CYP20A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16702-1-AP

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP20A1 fusion protein Ag10099
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2261395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CYP20A1 antibody 16702-1-APDownload protocol
IHC protocol for CYP20A1 antibody 16702-1-APDownload protocol
IP protocol for CYP20A1 antibody 16702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols