CXorf15 Polyclonal antibody

CXorf15 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF

Conjugate

Unconjugated

Publications

1

Cat no : 22357-1-AP

Synonyms

CXorf15, ELRG, FIAT, Gamma taxilin, LSR5, TXLNG, TXLNG,ELRG

---

Validation Data Gallery

• 

  WB analysis of mouse kidney using 22357-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22357-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22357-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22357-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 22357-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22357-1-AP (CXorf15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, Jurkat cells, MCF-7 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 22357-1-AP is CXorf15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXorf15 fusion protein Ag17936
• Full Name: chromosome X open reading frame 15
• Calculated Molecular Weight: 528 aa, 61 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC101576
• Gene Symbol: CXorf15
• Gene ID (NCBI): 55787
• RRID: AB_2879087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma