FLJ23584 Polyclonal antibody

FLJ23584 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21143-1-AP

Synonyms

C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 21143-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21143-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21143-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21143-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 21143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21143-1-AP

  Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, L02 cells, mouse liver tissue
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21143-1-AP is FLJ23584 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FLJ23584 fusion protein Ag15647
• Full Name: hypothetical FLJ23584
• Calculated Molecular Weight: 234 aa, 26 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC007210
• Gene Symbol: CTA-216E10.6
• Gene ID (NCBI): 79640
• RRID: AB_10732689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.