CRMP1 Polyclonal antibody

CRMP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29497-1-AP

Synonyms

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

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Validation Data Gallery

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  WB analysis using 29497-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29497-1-AP (CRMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29497-1-AP targets CRMP1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

• Tested Reactivity: Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRMP1 fusion protein Ag31559
• Full Name: collapsin response mediator protein 1
• Calculated Molecular Weight: 62KD
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: NM_001014809
• Gene Symbol: CRMP1
• Gene ID (NCBI): 1400
• RRID: AB_2918317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.