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Biotin-conjugated CRK Monoclonal antibody

CRK Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

ELISA

Conjugate

Biotin

CloneNo.

2B7E1

Cat no : Biotin-67035

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Validation Data Gallery

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  • ELISA experiment of Ag28793 using Biotin-conjugated CRK Monoclonal antibody (Biotin-67035)

    ELISA experiment of Ag28793 using Biotin-67035

    Biotin-67035 tested by ELISA. Ag28793(CRK 41-304 aa)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-67035 (start dilution 1:500. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate,terminated with 2M H2SO4,then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive ELISA detected inAg28793

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-67035 targets CRK in ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRK fusion protein Ag28793
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.