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COQ9 Polyclonal antibody
COQ9 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14874-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
14874-1-AP targets COQ9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COQ9 fusion protein Ag6645 |
| Full Name | coenzyme Q9 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC064946 |
| Gene Symbol | COQ9 |
| Gene ID (NCBI) | 57017 |
| RRID | AB_2082218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COQ9, also named as C16orf49, HSPC326, and PSEC0129, is a 318 amino acid protein, which belongs to the COQ9 family. COQ9 localizes in the Mitochondrion and is involved in the biosynthesis of coenzyme Q COQ9 has abilities to improve left ventricular performance and to reduce myocardial infarct size and cardiomyocyte apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ9 antibody 14874-1-AP | Download protocol |
| IHC protocol for COQ9 antibody 14874-1-AP | Download protocol |
| IF protocol for COQ9 antibody 14874-1-AP | Download protocol |
| IP protocol for COQ9 antibody 14874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness. | ||
J Am Soc Nephrol ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. | ||
J Med Genet Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes. | ||
Front Physiol Human COQ9 Rescues a coq9 Yeast Mutant by Enhancing Coenzyme Q Biosynthesis from 4-Hydroxybenzoic Acid and Stabilizing the CoQ-Synthome. | ||
Mol Genet Metab Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.
| ||
J Cell Biol Coenzyme Q biosynthetic proteins assemble in a substrate-dependent manner into domains at ER-mitochondria contacts. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (03-31-2021) | This antibody works well for our westerns of cultured mouse cell extracts.
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