COQ5 Polyclonal antibody

COQ5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17453-1-AP

Synonyms

COQ5

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 17453-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17453-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17453-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17453-1-AP (COQ5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17453-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17453-1-AP (COQ5 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below

Product Information

17453-1-AP targets COQ5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COQ5 fusion protein Ag10203
• Full Name: coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
• Calculated Molecular Weight: 327 aa, 37 kDa
• Observed Molecular Weight: 34.7 kDa
• GenBank Accession Number: BC107874
• Gene Symbol: COQ5
• Gene ID (NCBI): 84274
• RRID: AB_10547974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	J Exp Med; Implication of folate deficiency in CYP2U1 loss of function.; Authors - Claire Pujol
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
human	WB	J Am Soc Nephrol; ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.; Authors - Eugen Widmeier
mouse	WB	EMBO Mol Med; The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.; Authors - Marta Luna-Sánchez
mouse	WB	Sci Rep; Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.; Authors - María Rodríguez-Hidalgo