COMMD5 Monoclonal antibody

COMMD5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E10G6

Cat no : 67043-1-Ig

Synonyms

COMM domain containing 5, COMMD5, FLJ13008, HCARG, HT002



Tested Applications

Positive WB detected inhuman heart tissue, rat heart tissue, MCF-7 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67043-1-Ig targets COMMD5 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COMMD5 fusion protein Ag18464
Full Name COMM domain containing 5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC003055
Gene Symbol COMMD5
Gene ID (NCBI) 28991
RRIDAB_2882357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Protocols

Product Specific Protocols
WB protocol for COMMD5 antibody 67043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome

Authors - Michael D Healy
humanWB

Aging (Albany NY)

TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs)

Authors - Jianyun Liu