COMMD3 Polyclonal antibody

COMMD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26240-1-AP

Synonyms

BUP, C10orf8, COMM domain containing 3, COMMD3, Protein Bup, Protein PIL

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Validation Data Gallery

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  WB analysis of A549 using 26240-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26240-1-AP (COMMD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 26240-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 26240-1-AP (COMMD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thymus using 26240-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 26240-1-AP (COMMD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 26240-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 26240-1-AP (COMMD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, mouse thymus tissue
• Positive IHC detected in: human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26240-1-AP is COMMD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COMMD3 fusion protein Ag23968
• Full Name: COMM domain containing 3
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC022898
• Gene Symbol: COMMD3
• Gene ID (NCBI): 23412
• RRID: AB_2880440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang