CNKSR1 Monoclonal antibody

CNKSR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E11H8

Cat no : 66796-1-Ig

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 66796-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66796-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66796-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66796-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, HT-29 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66796-1-Ig targets CNKSR1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNKSR1 fusion protein Ag27855
• Full Name: connector enhancer of kinase suppressor of Ras 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC011604
• Gene Symbol: CNKSR1
• Gene ID (NCBI): 10256
• RRID: AB_2882140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.