CIDEC Polyclonal antibody

CIDEC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12287-1-AP

Synonyms

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27



Tested Applications

Positive WB detected inMCF-7 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIDEC fusion protein Ag2913
Full Name cell death-inducing DFFA-like effector c
Calculated Molecular Weight 238 aa, 27 kDa
Observed Molecular Weight 27-35 kDa
GenBank Accession NumberBC016851
Gene Symbol CIDEC
Gene ID (NCBI) 63924
RRIDAB_2877842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Protocols

Product Specific Protocols
WB protocol for CIDEC antibody 12287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Identification of potential pathogenic hepatic super-enhancers regulatory network in high-fat diet induced hyperlipidemia

Authors - Yingying Hu
humanWB

Commun Biol

Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.

Authors - Yi Jin
humanWB

Cancer Biol Ther

The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer

Authors - Wen-Jing Yun
humanWB

Biomed Pharmacother

Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.

Authors - Xingran Chen
mouseWB

Aging Cell

Long-term NAD+ supplementation prevents the progression of age-related hearing loss in mice

Authors - Mustafa N Okur
mouseWB

Cell Death Discov

Shift work promotes adipogenesis via cortisol-dependent downregulation of EGR3-HDAC6 pathway

Authors - Xinxing Wan