CIAO1 Polyclonal antibody

CIAO1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10295-1-AP

Synonyms

CIA1, CIAO1, WDR39

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Validation Data Gallery

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  WB analysis of HepG2 using 10295-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10295-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10295-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 10295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10295-1-AP

  Immunofluorescent analysis of HepG2 cells using 10295-1-AP (CIAO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells
• Positive IHC detected in: human small intestine tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10295-1-AP targets CIAO1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIAO1 fusion protein Ag0349
• Full Name: cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC001395
• Gene Symbol: CIAO1
• Gene ID (NCBI): 9391
• RRID: AB_2260509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIAO1, also named as CIA1 or WDR39, is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. It may also play a role in chromosome segregation. The predicted MW of this protein is 38 kDa. 10295-1-AP detected 48-55 kDa band because of phosphorylation. And 10295-1-AP do well in IHC and IF detection.