CHST14 Polyclonal antibody

CHST14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17749-1-AP

Synonyms

CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17749-1-AP targets CHST14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST14 fusion protein Ag12014
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
Calculated Molecular Weight 376 aa, 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC049214
Gene Symbol CHST14
Gene ID (NCBI) 113189
RRIDAB_2079051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHST14 antibody 17749-1-APDownload protocol
IHC protocol for CHST14 antibody 17749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Oncol

A comprehensive analysis of the expression, immune infiltration, prognosis and partial experimental validation of CHST family genes in gastric cancer

Authors - Jinbo Zhan