CERCAM Polyclonal antibody

CERCAM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16411-1-AP

Synonyms

CEECAM1, CERCAM, GLT25D3, KIAA1502

---

Validation Data Gallery

• 

  WB analysis using 16411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16411-1-AP (CERCAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse placenta using 16411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 16411-1-AP (CERCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16411-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16411-1-AP (CERCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 16411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 16411-1-AP (CERCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16411-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16411-1-AP (CERCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, U-87 MG cells, human placenta tissue
• Positive IHC detected in: mouse placenta tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

16411-1-AP targets CERCAM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CERCAM fusion protein Ag9352
• Full Name: cerebral endothelial cell adhesion molecule
• Calculated Molecular Weight: 517 aa, 59 kDa
• Observed Molecular Weight: 68 kDa, 48 kDa
• GenBank Accession Number: BC011811
• Gene Symbol: CERCAM
• Gene ID (NCBI): 51148
• RRID: AB_2077233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Mol Cancer; Multi-cohort validation of Ascore: an anoikis-based prognostic signature for predicting disease progression and immunotherapy response in bladder cancer; Authors - Tianlei Xie
human	WB,IHC	Cancer Med; The oncogenic role of the cerebral endothelial cell adhesion molecule (CERCAM) in bladder cancer cells in vitro and in vivo.; Authors - Yali Zuo