Cadherin-7 Polyclonal antibody

Cadherin-7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13598-1-AP

Synonyms

cadherin 7, type 2, Cadherin-7, CDH7, CDH7L1

---

Validation Data Gallery

• 

  WB analysis of A549 using 13598-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13598-1-AP (Cadherin-7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13598-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13598-1-AP (Cadherin-7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13598-1-AP (Cadherin-7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue, human prostate cancer tissue, human gliomas tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13598-1-AP targets Cadherin-7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cadherin-7 fusion protein Ag4518
• Full Name: cadherin 7, type 2
• Calculated Molecular Weight: 785 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC036786
• Gene Symbol: CDH7
• Gene ID (NCBI): 1005
• RRID: AB_2078256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Nanomedicine; VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis; Authors - Yi-Fang Wu