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CDCA7 Polyclonal antibody
CDCA7 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15249-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15249-1-AP targets CDCA7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDCA7 fusion protein Ag4297 |
| Full Name | cell division cycle associated 7 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC027966 |
| Gene Symbol | CDCA7 |
| Gene ID (NCBI) | 83879 |
| RRID | AB_2878119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IF protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IP protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses | ||
Proc Natl Acad Sci U S A HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome. | ||
J Exp Med Loss of ZBTB24 impairs nonhomologous end-joining and class-switch recombination in patients with ICF syndrome. | ||
Hum Mol Genet Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals. | ||
Bioorg Chem Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation | ||
J Biol Chem TET2-BCLAF1 transcription repression complex epigenetically regulates the expression of colorectal cancer gene Ascl2 via methylation of its promoter. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH XIAOBING (Verified Customer) (11-05-2019) | CDCA7 antibody was used to stain the 293T cells transfected with human CDCA7 plasmid. Overexpressed CDCA7 (Red) was localized in the nucleus (DAPI was used to label the nucleus).
 |
FH Guillaume (Verified Customer) (09-08-2019) | I used cells mutated for ZBTB24 which control CDCA7 expression to test the specificity of the antibody. In WB and IP, the signal seem to be lower than the expected size in kDa. This CDCA7 antibody is not very efficient in ChIP
|
