CDCA5 Polyclonal antibody

CDCA5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29316-1-AP

Synonyms

CDCA5, p35, SORORIN

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Validation Data Gallery

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  WB analysis using 29316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29316-1-AP (CDCA5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, LNCaP cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29316-1-AP targets CDCA5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDCA5 fusion protein Ag30966
• Full Name: cell division cycle associated 5
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27~35 kDa
• GenBank Accession Number: BC011000
• Gene Symbol: CDCA5
• Gene ID (NCBI): 113130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).