CDC16/APC6 Polyclonal antibody

CDC16/APC6 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14307-1-AP

Synonyms

ANAPC6, APC6, CDC16, CDC16 homolog, CDC16/APC6, CDC16Hs, Cyclosome subunit 6

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Validation Data Gallery

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  WB analysis using 14307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14307-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14307-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14307-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14307-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 14307-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14307-1-AP

  Immunofluorescent analysis of Hela cells, using CDC16 antibody 14307-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, Jurakt cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14307-1-AP targets CDC16/APC6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC16/APC6 fusion protein Ag5427
• Full Name: cell division cycle 16 homolog (S. cerevisiae)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC010875
• Gene Symbol: CDC16
• Gene ID (NCBI): 8881
• RRID: AB_2074638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.