CDADC1 Polyclonal antibody

CDADC1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28169-1-AP

Synonyms

bA103J18.1, CDADC1, NYD SP15

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Validation Data Gallery

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  IHC staining of human testis using 28169-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 28169-1-AP (CDADC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28169-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28169-1-AP (CDADC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Saos-2 using 28169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of Saos-2 using 28169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of Saos-2 using 28169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Saos-2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28169-1-AP targets CDADC1 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDADC1 fusion protein Ag27374
• Full Name: cytidine and dCMP deaminase domain containing 1
• Calculated Molecular Weight: 514 aa, 58 kDa
• GenBank Accession Number: BC125202
• Gene Symbol: CDADC1
• Gene ID (NCBI): 81602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDADC1 (cytidine and dCMP deaminase domain containing 1) catalyzes the deamination of cytidine and deoxycytidine into uridine and deoxyuridine, respectively, and may play an important role in testicular development and spermatogenesis (PMID: 26945630, 16955368).