CCT7 Polyclonal antibody

CCT7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15994-1-AP

Synonyms

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta



Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15994-1-AP targets CCT7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT7 fusion protein Ag8868
Full Name chaperonin containing TCP1, subunit 7 (eta)
Calculated Molecular Weight 543 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC019296
Gene Symbol CCT7
Gene ID (NCBI) 10574
RRIDAB_2073903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Protocols

Product Specific Protocols
WB protocol for CCT7 antibody 15994-1-APDownload protocol
IF protocol for CCT7 antibody 15994-1-APDownload protocol
IP protocol for CCT7 antibody 15994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
WB

Nat Commun

Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.

Authors - Adela S Oliva Chávez
humanIHC

Aging (Albany NY)

Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.

Authors - Xinghua Huang
mouseWB

Virology

Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.

Authors - Sandhya Gopal
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta
humanWB

Clin Proteomics

Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis

Authors - Hongyu Chen