CoraLite® Plus 488-conjugated CCT2 Monoclonal antibody

CCT2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rabbit, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H6C7

Cat no : CL488-68214

Synonyms

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-68214

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 CCT2 antibody (CL488-68214, Clone: 2H6C7 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL488-68214

  1X10^6 NIH/3T3 cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human CCT2 (CL488-68214, Clone:2H6C7) (red), or 1 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68214 targets CCT2 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.

• Tested Reactivity: Human, Mouse, Pig, Rabbit, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT2 fusion protein Ag19264
• Full Name: chaperonin containing TCP1, subunit 2 (beta)
• Calculated Molecular Weight: 535 aa, 57 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC113516
• Gene Symbol: CCT2
• Gene ID (NCBI): 10576
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)