Cyclon/CCDC86 Polyclonal antibody

Cyclon/CCDC86 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14947-1-AP

Synonyms

CCDC86, CYCLON, Cyclon/CCDC86

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Validation Data Gallery

• 

  WB analysis of mouse stomach using 14947-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14947-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14947-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14947-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14947-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14947-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14947-1-AP

  Immunofluorescent analysis of HepG2 cells using 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse stomach tissue, HepG2 cells, human heart tissue, human liver tissue, human stomach tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14947-1-AP targets Cyclon/CCDC86 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclon/CCDC86 fusion protein Ag6754
• Full Name: coiled-coil domain containing 86
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC001378
• Gene Symbol: CCDC86
• Gene ID (NCBI): 79080
• RRID: AB_2072318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.