CEP89, CCDC123 Monoclonal antibody

CEP89, CCDC123 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F12C5

Cat no : 68112-1-Ig

Synonyms

CCDC123, FLJ14640

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Validation Data Gallery

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  WB analysis of Neuro-2a using 68112-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CEP89, CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

• 

  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Neuro-2a cells, A431 cells, SH-5Y5Y cells, U-251 cells, SH-SY5Y cells
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68112-1-Ig targets CEP89, CCDC123 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP89, CCDC123 fusion protein Ag28339
• Full Name: coiled-coil domain containing 123
• Calculated Molecular Weight: 783 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC136328
• Gene Symbol: CCDC123
• Gene ID (NCBI): 84902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).