CAPN2 Monoclonal antibody

CAPN2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E1F10

Cat no : 66977-1-Ig

Synonyms

Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain

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Validation Data Gallery

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  WB analysis using 66977-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66977-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66977-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66977-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66977-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66977-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66977-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66977-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66977-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66977-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, LNCaP cells, HEK-293 cells, rat brain tissue, mouse brain tissue, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue, human stomach cancer tissue, human colon cancer tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66977-1-Ig targets CAPN2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPN2 fusion protein Ag28427
• Full Name: calpain 2, (m/II) large subunit
• Calculated Molecular Weight: 700 aa, 80 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC021303
• Gene Symbol: CAPN2
• Gene ID (NCBI): 824
• RRID: AB_2882297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928, and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from the human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.; Authors - Fengming Shen
human	IF	Cell Mol Life Sci; Calpains as novel players in the molecular pathogenesis of spinocerebellar ataxia type 17.; Authors - Jonasz Jeremiasz Weber