CAPG Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CAPG Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A5B2

Cat no : 66277-1-PBS

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Validation Data Gallery

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  WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 66277-1-Ig (same clone as 66277-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66277-1-PBS targets CAPG in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPG fusion protein Ag24676
• Full Name: capping protein (actin filament), gelsolin-like
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-40 kDa
• GenBank Accession Number: BC000728
• Gene Symbol: CAPG
• Gene ID (NCBI): 822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)