C8orf4 Polyclonal antibody

C8orf4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26279-1-AP

Synonyms

C8orf4, hTC 1, TC 1, TC1, Thyroid cancer protein 1, Uncharacterized protein C8orf4

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Validation Data Gallery

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  WB analysis of mouse kidney using 26279-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26279-1-AP (C8orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26279-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26279-1-AP (C8orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 26279-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26279-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 26279-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26279-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IHC detected in: human thyroid cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 26279-1-AP is C8orf4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C8orf4 fusion protein Ag23930
• Full Name: chromosome 8 open reading frame 4
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC021672
• Gene Symbol: C8orf4
• Gene ID (NCBI): 56892
• RRID: AB_2880460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Medicine (Baltimore); Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma; Authors - Gang Ren