C7orf30 Polyclonal antibody

C7orf30 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 22838-1-AP

Synonyms

C7orf30, MALSU1

---

Validation Data Gallery

• 

  WB analysis using 22838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22838-1-AP (C7orf30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22838-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22838-1-AP (C7orf30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22838-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22838-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22838-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22838-1-AP (C7orf30 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, U-251 cells
• Positive IHC detected in: human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

22838-1-AP targets C7orf30 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C7orf30 fusion protein Ag18286
• Full Name: chromosome 7 open reading frame 30
• Calculated Molecular Weight: 234 aa, 26 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC012331
• Gene Symbol: C7orf30
• Gene ID (NCBI): 115416
• RRID: AB_11182483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf30, also known as MALSU1, is a member of the iojap protein family. It was predicted to enable ribosomal large subunit binding activity. It was Involved in negative regulation of mitochondrial translation and ribosomal large subunit biogenesis.

Publications

Species	Application	Title
human	WB	Nat Commun; Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.; Authors - Hauke S Hillen
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Cell Rep; The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.; Authors - Ya-Ting Tu
human	WB	bioRxiv; Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival; Authors - Conor T Ronayne
human	WB	EMBO Rep; Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival; Authors - Conor T Ronayne