C6orf15 Polyclonal antibody

C6orf15 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24953-1-AP

Synonyms

C6orf15, Protein STG, STG

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Validation Data Gallery

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  WB analysis using 24953-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 24953-1-AP (C6orf15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 24953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 24953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 24953-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using C6orf15 antibody (24953-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

24953-1-AP targets C6orf15 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C6orf15 fusion protein Ag20276
• Full Name: chromosome 6 open reading frame 15
• Calculated Molecular Weight: 325 aa, 34 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC153157
• Gene Symbol: C6orf15
• Gene ID (NCBI): 29113
• RRID: AB_2879818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.

Publications

Species	Application	Title
human	IHC	Pathol Res Pract; C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer; Authors - Xiaoyu Xiong