C5orf4 Polyclonal antibody

C5orf4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22046-1-AP

Synonyms

C5orf4, fatty acid hydroxylase domain containing 2, FAXDC2, Uncharacterized protein C5orf4

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 22046-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22046-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22046-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, A549 cells, HepG2 cells
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 22046-1-AP is C5orf4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C5orf4 fusion protein Ag16960
• Full Name: chromosome 5 open reading frame 4
• Calculated Molecular Weight: 333 aa, 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC007216
• Gene Symbol: C5orf4
• Gene ID (NCBI): 10826
• RRID: AB_2878979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid hydroxylase domain containing 2 (FAXDC2), also known as C5orf4, is a 40 kDa protein. The function of FAXDC2 remains largely unknown. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

Publications

Species	Application	Title
human	WB	Blood Cancer J; Novel function of FAXDC2 in megakaryopoiesis.; Authors - Q Jin
mouse	WB	J Clin Invest; The cholesterol biosynthesis enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling; Authors - Babita Madan