C3orf20 Polyclonal antibody

C3orf20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20906-1-AP

Synonyms

C3orf20

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Validation Data Gallery

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  WB analysis of human brain using 20906-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20906-1-AP (C3orf20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20906-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20906-1-AP (C3orf20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20906-1-AP

  IP result of anti-C3orf20 (IP:20906-1-AP, 3ug; Detection:20906-1-AP 1:500) with mouse brain tissue lysate 10000ug.

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  IHC staining of human liver using 20906-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20906-1-AP (C3orf20 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20906-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20906-1-AP (C3orf20 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20906-1-AP is C3orf20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C3orf20 fusion protein Ag15031
• Full Name: chromosome 3 open reading frame 20
• Calculated Molecular Weight: 904 aa, 101 kDa
• Observed Molecular Weight: 101 kDa, 88 kDa
• GenBank Accession Number: BC038406
• Gene Symbol: C3orf20
• Gene ID (NCBI): 84077
• RRID: AB_10732599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C3orf20 has not been widely studied, and is yet to be fully elucidated. Two isoforms were produced by alternative splicing with predicted MW of 88 and 101 kDa according to UniProt. Catalog#20906-1-AP can recognize both 88 and 101 kDa isoforms.