C2orf3 Polyclonal antibody

C2orf3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26029-1-AP

Synonyms

C2orf3, DNABF, GCF, TCF 9, TCF9, Transcription factor 9

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Validation Data Gallery

• 

  WB analysis of PC-3 using 26029-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26029-1-AP (C2orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26029-1-AP is C2orf3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf3 fusion protein Ag23315
• Full Name: chromosome 2 open reading frame 3
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC064559
• Gene Symbol: C2orf3
• Gene ID (NCBI): 6936
• RRID: AB_2880343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GC-rich sequence DNA-binding factor is a protein that in humans is encoded by the C2orf3 gene. C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. The MW of this protein is 89 kDa, and this antibody specially recognises the 89 kDa protein.