TMEM230 Monoclonal antibody

TMEM230 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4D3A7

Cat no : 67247-1-Ig

Synonyms

C20orf30, dJ1116H23.2.1, HSPC274, TMEM230, UNQ2432/PRO4992

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Validation Data Gallery

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  WB analysis using 67247-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67247-1-Ig (TMEM230 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67247-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67247-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67247-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67247-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67247-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67247-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67247-1-Ig (C20orf30 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, Caco-2 cells, HT-29 cells, A549 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67247-1-Ig targets TMEM230 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM230 fusion protein Ag15080
• Full Name: chromosome 20 open reading frame 30
• Calculated Molecular Weight: 183 aa, 20 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC011990
• Gene Symbol: C20orf30
• Gene ID (NCBI): 29058
• RRID: AB_2882524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.