TEX38 Polyclonal antibody

TEX38 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24429-1-AP

Synonyms

ATPAF1-AS1, C1orf223, TEX38

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Validation Data Gallery

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  WB analysis of mouse testis using 24429-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24429-1-AP (TEX38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 24429-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24429-1-AP (TEX38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24429-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24429-1-AP (TEX38 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24429-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24429-1-AP (TEX38 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, MDA-MB-453s cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 24429-1-AP is TEX38 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX38 fusion protein Ag19833
• Full Name: chromosome 1 open reading frame 223
• Calculated Molecular Weight: 206 aa, 23 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC126318
• Gene Symbol: C1orf223
• Gene ID (NCBI): 374973
• RRID: AB_2879544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX38, also named as ATPAF1-AS1 and C1orf223, is ATPAF1 antisense gene protein 1. This antibody detects 25-28 kDa with mouse testis and MDA-MB-453s cell lysate.

Publications

Species	Application	Title
human,mouse	IF	Adv Sci (Weinh); Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells; Authors - Yidong Chen