C1orf2 Polyclonal antibody

C1orf2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27969-1-AP

Synonyms

C1orf2, COTE1, FAM189B, Protein COTE1, Protein FAM189B

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Validation Data Gallery

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  WB analysis using 27969-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27969-1-AP (C1orf2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27969-1-AP targets C1orf2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf2 fusion protein Ag27428
• Full Name: chromosome 1 open reading frame 2
• Calculated Molecular Weight: 669 aa, 71 kDa
• Observed Molecular Weight: 69-71 kDa
• GenBank Accession Number: BC006493
• Gene Symbol: C1orf2
• Gene ID (NCBI): 10712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1orf2 is also named COTE1, FAM189B and ENTREP3. C1orf2 is identified as a potential binding partner of a WW domain-containing protein which is involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants. COTE1 contributes to human hepatocarcinogenesis by regulating cell proliferation through the modulation of WWOX signaling, and it upregulated in hepatocellular carcinoma specimens and HCC cell lines (PMID: 23317259, PMID: 24899407).