COA7 Polyclonal antibody

COA7 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25361-1-AP

Synonyms

C1orf163, COA7, RESA1, Sel1 repeat-containing protein 1, SELRC1

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Validation Data Gallery

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  WB analysis using 25361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25361-1-AP (COA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25361-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25361-1-AP (COA7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 25361-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 25361-1-AP (C1orf163 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, COLO 320 cells, PC-3 cells
• Positive IHC detected in: human placenta tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25361-1-AP targets COA7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COA7 fusion protein Ag19734
• Full Name: chromosome 1 open reading frame 163
• Calculated Molecular Weight: 231 aa, 26 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC015313
• Gene Symbol: C1orf163
• Gene ID (NCBI): 65260
• RRID: AB_2880043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase assembly factor 7 (COA7) is a 231 aa long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins (PMID: 30885959). COA7 is a metazoan-specific assembly factor, the absence or mutation of which in humans accompanies complex IV assembly defects and neurological conditions (PMID: 35210360). COA7 has a molecular weight of ~25 kDa.

Publications

Species	Application	Title
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons
human	WB	Mol Biol Cell; Discovery of bactericides as an acute mitochondrial membrane damage inducer.; Authors - Ryan Houston