C19orf36 Polyclonal antibody

C19orf36 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25650-1-AP

Synonyms

C19orf36, IMAGE:4215339, IZUMO4, Sperm 22 kDa protein c113

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Validation Data Gallery

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  WB analysis of mouse testis using 25650-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25650-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25650-1-AP (C19orf36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 25650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 25650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 25650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25650-1-AP (C19orf36 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25650-1-AP

  Immunofluorescent analysis of HepG2 cells using 25650-1-AP (C19orf36 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25650-1-AP is C19orf36 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf36 fusion protein Ag20434
• Full Name: chromosome 19 open reading frame 36
• Calculated Molecular Weight: 232 aa, 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC014609
• Gene Symbol: C19orf36
• Gene ID (NCBI): 113177
• RRID: AB_2880175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IZUMO4, also named as C19orf36, is sperm 22 kDa protein c113. It has 4 isoforms with MW 17-27 kDa.

Publications

Species	Application	Title
bovine	IF,WB	Int J Biol Macromol; TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.; Authors - Na Wang