Tim21 Polyclonal antibody

Tim21 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16641-1-AP

Synonyms

C18orf55, HSPC154, Mitochondrial import inner membrane translocase subunit Tim21, TIM21, TIMM21

---

Validation Data Gallery

• 

  IHC staining of human liver using 16641-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16641-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16641-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16641-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16641-1-AP (Tim21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human liver tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16641-1-AP targets Tim21 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tim21 fusion protein Ag10002
• Full Name: chromosome 18 open reading frame 55
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC000892
• Gene Symbol: C18orf55
• Gene ID (NCBI): 29090
• RRID: AB_2062082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Mitochondrial protein sorting as a therapeutic target for ATP synthase disorders.; Authors - Raeka S Aiyar
human	WB, IF	Int J Gynecol Cancer; Establishment of paclitaxel-resistant cell line and the underlying mechanism on drug resistance.; Authors - Jingjing Zhang