C14orf94 Polyclonal antibody

C14orf94 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20115-1-AP

Synonyms

C14orf94, HAUS4

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Validation Data Gallery

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  WB analysis of PC-3 using 20115-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20115-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20115-1-AP (C14orf94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 20115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 20115-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 20115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 20115-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20115-1-AP (C14orf94 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse skin tissue
• Positive IHC detected in: human lung tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20115-1-AP is C14orf94 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C14orf94 fusion protein Ag13986
• Full Name: chromosome 14 open reading frame 94
• Calculated Molecular Weight: 363 aa, 42 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001916
• Gene Symbol: C14orf94
• Gene ID (NCBI): 54930
• RRID: AB_10638430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.