C14orf28 Polyclonal antibody

C14orf28 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 26130-1-AP

Synonyms

c14_5270, C14orf28, DRIP1

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Validation Data Gallery

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  WB analysis of HeLa using 26130-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26130-1-AP (C14orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26130-1-AP

  IP result of anti-C14orf28 (IP:26130-1-AP, 4ug; Detection:26130-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IF Staining of HeLa using 26130-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26130-1-AP (C14orf28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26130-1-AP is C14orf28 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C14orf28 fusion protein Ag23441
• Full Name: chromosome 14 open reading frame 28
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC105030
• Gene Symbol: C14orf28
• Gene ID (NCBI): 122525
• RRID: AB_2880393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.