NRDE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NRDE2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24968-1-AP

Synonyms

C14orf102, NRDE2, Protein NRDE2 homolog, UPF0614 protein C14orf102

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 24968-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24968-1-AP (C14orf102 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 24968-1-AP

  IP result of anti-NRDE2 (IP:24968-1-AP, 5ug; Detection:24968-1-AP 1:500) with MCF-7 cells lysate 1200ug.

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 24968-1-AP is NRDE2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRDE2 fusion protein Ag21805
• Full Name: chromosome 14 open reading frame 102
• Calculated Molecular Weight: 1164 aa, 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC118673
• Gene Symbol: C14orf102
• Gene ID (NCBI): 55051
• RRID: AB_2879825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP,IP	Genes Dev; An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression.; Authors - Koichi Ogami
human	WB,chIP	RNA Biol; NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells.; Authors - Patricia Richard
human	WB	J Virol; Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.; Authors - Julio C Ruiz ; KD Validated
human	WB,IP	Genes Dev; NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.; Authors - Jianshu Wang ; KD Validated