C11orf67 Polyclonal antibody

C11orf67 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20414-1-AP

Synonyms

AAMDC, C11orf67, CK067, PTD015, UPF0366 protein C11orf67

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Validation Data Gallery

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  WB analysis of mouse testis using 20414-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20414-1-AP (C11orf67 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20414-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20414-1-AP (C11orf67 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20414-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20414-1-AP (C11orf67 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 20414-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20414-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, MCF-7 cells, rat testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20414-1-AP is C11orf67 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C11orf67 fusion protein Ag14258
• Full Name: chromosome 11 open reading frame 67
• Calculated Molecular Weight: 122 aa, 13 kDa
• GenBank Accession Number: BC002752
• Gene Symbol: C11orf67
• Gene ID (NCBI): 28971
• RRID: AB_10694438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.