BUD13 Polyclonal antibody

BUD13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20163-1-AP

Synonyms

BUD13, BUD13 homolog, BUD13 homolog (S. cerevisiae), fSAP71

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Validation Data Gallery

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  WB analysis using 20163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20163-1-AP (BUD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 20163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20163-1-AP targets BUD13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BUD13 fusion protein Ag13665
• Full Name: BUD13 homolog (S. cerevisiae)
• Calculated Molecular Weight: 619 aa, 71 kDa
• Observed Molecular Weight: 71-85 kDa
• GenBank Accession Number: BC006350
• Gene Symbol: BUD13
• Gene ID (NCBI): 84811
• RRID: AB_2878650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BUD13 homolog (BUD13) is a component of the retention and splicing (RES) complex which was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. As an RNA binding protein (RBP), BUD13 expression is associated with poor prognosis and may be involved in the development and progression of hepatocellular carcinoma (PMID: 37605593).