BLVRA Monoclonal antibody

BLVRA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10F2

Cat no : 68175-1-Ig

Synonyms

Biliverdin IX alpha reductase, biliverdin reductase A, BLVR, BLVRA, BVR, BVR A, BVRA

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Validation Data Gallery

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  WB analysis using 68175-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68175-1-Ig (BLVRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: L02 cells, DU 145 cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68175-1-Ig targets BLVRA in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BLVRA fusion protein Ag32730
• Full Name: biliverdin reductase A
• Calculated Molecular Weight: 33.2 kDa
• Observed Molecular Weight: 37-40 kDa, 66 kDa
• GenBank Accession Number: BC008456
• Gene Symbol: BLVRA
• Gene ID (NCBI): 644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLVRA(Biliverdin reductase A) is also named BLVR, BVR, and belongs to the biliverdin reductase subfamily. It catalyzes the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. This protein can exist as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).